DNA Replication
•Semi-conservative replication
–Half of double
helix made.
–Half is
conserved
–DNA polymerase
Meselson
& Stahl Experiment
–Grew E. coli on
N15 (heavy isotope) à heavy DNA
–Switched to N14
–Differential
centrifugation (caesium chloride gradient)
–Measured position
of DNA by UV260
–Checked in
subsequent generations